Phytochemical study and antioxidant activity of the heartwoods of artocarpus scortechinii king

Abstract

Artocarpus scortechinii belongs to Moraceae family or mulberry family. There are around 50 species of Artocarpus present in South East Asia and Pacific Island which 20 species can found in Malaysia. Phytochemical study and antioxidant activity of the heartwoods of A. scortechinii have been carried out. The extraction process was conducted using cold extraction method with three different polarity of solvent which were n-hexane, dichloromethane and methanol. Fractionation of the methanol extract was performed using vacuum liquid chromatography while purification of fractions was carried out using column chromatography. Two pure compounds had been successfully isolated from the methanol extract. The structure of pure compounds was determined by proton nuclear magnetic resonance (¹H NMR), ¹H-¹H COSY and FTIR spectroscopies. The compounds were identified as cudraflavone A and 4′,5-dihydroxy-6,7-(2,2-dimethylpyrano)-2′-methoxy-8-(3,3-dimethylallyl)flavone. Evaluation on the antioxidant activity of the crude extracts and pure compounds was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Both pure compounds were observed as inactive antioxidant agent.