Determination of Iron (Fe) and Lead (Pb) in Larvae using Atomic Absorption Spectroscopy (AAS)

Aida Amirah Mohd Salleh, Hashim Baharin

Abstract


The effect of rapid population causes the source of food to decrease especially in animal feed which makes the consumers need to purchase higher in order to get the best quality food for their pet. Some of animals feed may contain heavy metals that come from the production process in order to reduce cost. In this study, larvae of black soldier flies were used, as it is believed could be economical, low cost and acting as essential decomposers in breaking down organic substrates and returning nutrients to soil. The eggs of black soldier fly were divided into a beaker that contained chicken livers. 5-, 10- and 15-days age of larvae were collected to determine Pb and Fe contents. The larvae were heated with sulphuric acid (H2SO4) and nitric acid (HNO3) until they were totally digested. Lead (Pb) and iron (Fe) were analysed using atomic absorption spectroscopy (AAS). Chicken livers contained (0.426 ± 0.029) mg/g of Fe and (0.029 ± 0.015) mg/g of Pb. After testing and comparing the results, the concentration of lead in the larvae decreased while concentration of iron increased after eating the chicken livers. The larvae contained (0.135 ± 0.007) mg/g of Fe with 31.7% of Fe per 1 g of chicken liver in 5 days, (0.202 ± 0.009) mg/g of Fe with 47.3% of Fe per 1 g of chicken liver in day 10 and (0.247 ± 0.016) mg/g of Fe with 57.9% of Fe per 1 g of chicken liver in day 15 respectively. The larvae contained (0.027 ± 0.010) mg/g of Pb with 94.6% of Pb per 1 g of chicken liver in day 5, (0.012 ± 0.008) mg/g of Pb with 43.3% of Pb per 1 g of chicken liver in day 10 and (0.011 ± 0.005) mg/g of Pb with 38.5% of Pb per 1 g of chicken liver in day 15 respectively. In conclusion, the bigger the size of the larvae of black soldier fly, the higher the concentration of Fe and the lower the concentration of Pb in the larvae.

Keywords


digested; chicken liver; iron; lead

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