Phytochemical Screening of Antimicrobial Compounds in Unnamed Plant from Borneo by Using Percolation and GC-MS

Nur Syafiqah Nadiah Mohammad Rafi, Anthony Nyangson Steven

Abstract


The present study was carried out to identify the presence of potential antibacterial and antifungal agents in the leaves of an unnamed plant extracts by using GC-MS (Agilent 7820A GC System with 5977E MSD) subjected to cold percolation technique. Qualitatively phytochemical screening of leaves ethanolic extracts revealed the existence for both major and minor phytoconstituents. The identified bioactive compounds were matched with National Institute of Standards and Technology (NIST) library yield the selected compounds of 2-phenylbenzothiazole, bamethan, lysergamide, oxazepam, thimerosal, 2,4-di-tert-butylphenol, 2’-hydroxychalcone, 2,5-dihydroxybenzoic acid, thymol, and phytol. These different active compounds were classified according to their nature and its roles of biological activities as antibacterial and antifungal. 2-phenylbenzothiazole is potential as antibacterial properties, meanwhile 2,4-di-tert-butylphenol and phytol are potential as antimicrobial properties. Thymol and thimerosal revealed the existence as antibacterial and antifungal agent. The finding justifies its use in traditional medicine to treat various diseases.

Keywords


Plant; percolation; bioactive; antibacterial, antifungal

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