Qualitative Analysis using GC-MS and Maceration of Antifungal and Antioxidant Potential in an Indigenous Plant from Borneo

Abstract

The unnamed indigenous plant from Borneo were used in this project is known to have medicinal properties by natives for treatment of certain illness. The compounds were extracted by maceration method and further analysis of bioactive compounds (antioxidant and antifungal) by gas chromatography coupled with mass spectrometry (GC-MS, Agilent 7890 GC with 5977A MSD). The molecular weight and structure of the compounds of test material were ascertained by interpretation of the mass spectrum of GC-MS using the database of National Institute Standard and Technology (NIST). In this work, twenty three phytochemicals were analysed by GC-MS technique. Nine bioactive compounds were positively identified which are phytol, phthalic acid, 2-thiobarbituric acid, 2,5-bis(1,1-dimethylethyl)phenol, alpha-cholestan-2-one, 2,5- dihydroxybenzoic acid, 2,4- di-tert-butylphenol, dibutyl phthalate and neophytadiene. Phytol and gentisic acid are known to have antioxidant properties while dibutyl phthalate is known as an antifungal agent. This analysis revealed that contains unnamed plant extract mainly dibutyl phthalate. Therefore, it should be concluded that this plant is a potential candidate as a phytopharmaceutical plant