Fabrication and Characterization of Electrospun Polyvinyl Alcohol/Poly-ε-caprolactone Antibacterial Agent Composite Nanofiber for Potential Scaffold

Nur Ain Atiqah Mohd Amin, Abdull Rahim Mohd Yusoff, Yong Chee Tan

Abstract


Bacterial infection and injury caused by dressing removal are two concerning issues which affect the healing process in treatment of skin damagesd. Attenuated total reflection (ATR) shows that the spectra of polyvinyl alcohol/poly-ε-caprolactone (PVA/PCL) blend is very similar to the spectra of both pure PVA and PCL especially for the absorption of hydroxyl group and carbonyl group which contribute to PVA and PCL groups respectively. In this study, the electrospun of PVA/PCL nanofiber was coated with three different types of antibacterial agents which are poly-L-lysine, chlorhexidine gluconate and chitosan. The effects of three antibacterial agents were examined by Disk Diffusion Test against S.aureus and E. coli. The zone of inhibition was evaluated by measuring the diameter of the bacterial growth inhibition zone around the membrane. The results show that chlorhexidine gluconate is the best antibacterial agents to supress the growth of the S. aureus and E. coli. The field emission scanning electron microscopy (FESEM) images showed an increase in fiber diameter with the presence of the three antibacterial agents. Besides, corresponding tensile strength values for the electrospun of PVA/PCL/antibacterial agents tested appear to be within the range of tensile strength values for human skin which conclude that the electrospun of PVA/PCL/antibacterial agent nanofiber is suitable for regenerating human skin. Last but not least, the hydrophilicity of the electrospun of PVA/PCL/antibacterial agents nanofiber was confirmed by water contact angle measurement which is 0° and it is theoretically can improve the cell adhesion onto the membrane.

Keywords


Polyvinyl alcohol; poly-ε-caprolactone; antibacterial agent; electrospinning; scaffold

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