Qualitative and Quantitative Analysis of Anti-hypertensive Drug Atenolol with Coffee

Abstract

Normally, sufferers of high blood pressure and elderly also consumed coffee as beverages during taking of atenolol drugs. Coffee on the other hand is also a stimulant to the central nervous system which can keep them alert. In this study, UV-Vis and FTIR-ATR spectroscopy method were used for qualitative and quantitative analysis of caffeine towards atenolol consumption. From the UV-Vis spectrum, the relationship between the concentrations and the absorbance exhibited good linearity with correlation coefficient 0.9924 for atenolol and 0.9989 for caffeine at 273 nm wavelength. The calculated amount of lowest caffeine concentration was 2.15 ± 0.04 μg/mL and the percentage of error in caffeine was obtained at 1.7%. In atenolol sample, the concentration was obtained at 56.36 ± 0.11 μg/mL with 0.19% percentage error. At wavelength of 273 ± 1 nm, the 60 μg/mL atenolol solution mixed with 2, 4, 6, 8, 10 and 12 μg/mL standard caffeine after 15 minutes of stirring at room temperature displayed the increasing relationship. Further analysis by using FTIR-ATR showed 3 different peaks peaking at 1736 cm-1, 1365 cm-1 and 1217 cm-1 consecutively in 4 and 6 μg/mL caffeine added. In comparison with 60 μg/mL atenolol solution, the 3 main bands were detected at 3325 cm-1, 2109 cm-1 and 1636 cm-1